Due to some recent browser updates, all modern web browsers have discontinued auto-playing media in web pages. Similarly, HTML5 and responsive courses created in Captivate will not auto-play. Autoplaying is not honored even after enabling the option Auto Play in Preferences > Project > Start and End.
While SWF videos will continue to auto-play, HTML5 and other responsive courses will not auto-play.
Even though auto-playing of media was not possible in devices, now the same behavior is extended to desktop browsers.
We recommend that you leave the Auto Play option disabled, since it does not get honored.